Investigation of Antimicrobial Activities and 16S rRNA Sequences of Actinomycetes Isolated from Karst Caves in the Eastern Black Sea Region of Türkiye

ABSTRACT 
 Considering that most of the antibiotics originate from actinomycete group bacteria, especially Streptomyces genus, it is predicted novel actinomycetes isolated extreme environments such as caves may bring to medical world. The study aimed screen antimicrobial activity three karst in Türkiye and identify selected isolates with by molecular methods. One hundred seventy-nine Akçakale, Kırklar (Altıntaş), Köprübaşı Caves Gümüşhane province Eastern Black Sea Region were included study. was investigated using modified cross-streak agar method against seven Gram-negative Gram-positive one yeast strain. 16S ribosomal RNA sequencing performed for identification phylogenetic analysis isolates. Fifty-three (29.6%) displayed at least tested microorganisms. rate exhibiting Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Chromobacterium violaceum, Klebsiella pneumoniae, Salmonella Typhimurium, Escherichia coli, Acinetobacter haemolyticus, Pseudomonas aeruginosa, Enterobacter aerogenes, Candida albicans 21.2%, 20.0%, 16.8%, 12.8%, 3.4%, 2.8%, 2.2%, 1.1%, 0.6%, respectively. An isolate, TRMS 124, showed 10 different test rRNA sequence 26 randomly among microorganisms performed, 24 identified spp. Others Embleya scabrispora Couchioplanes caeruleus. These results could be good sources isolating potential produce compounds.